Biomarker Discovery:Article Title: P-selectin mobility undergoes a sol-gel transition as it diffuses from exocytosis sites into the cell membrane
Article Snippet: .. Antibody reagents and Validation Species Manufacturer Clone / catalogue number Dilutions used for ICC or WB 1) VWF Rabbit pAb DAKO/Agilent Santa Clara, CA USA A0082 1:10000 2) VWF Sheep pAb Serotec Raleigh, NC, USA AHP062 1:10000 3) P-selectin Mouse pAb Bio-Rad (formerly AbD Serotec) AK6/MCA796 1:50 4) Control mouse IgG Mouse pAb Invitrogen/ThermoFisher 16-4714-82 1:50 5) Heparan sulphate Rabbit mAb Sigma-Aldrich MAB1948P/A7L6 1:500 6) Chondroitin-4sulphate Mouse mAb Sigma-Aldrich MAB2030/clone BE-123 1:50 7) FITC-WGA - Vector Labs Inc Burlingame, CA, USA FL-1021 1:400 8) anti-GFP Rabbit mAb Invitrogen/ThermoFisher A-6455 1:300 9) anti-GFP Sheep pAb Bio-Rad Labs Watford, UK 4745-1051 1:250 10) AP2alpha Mouse mAb Invitrogen/ThermoFisher AP6/ MA1-064 1:400 11) anti RFP Rabbit pAb Abcam Cambridge, UK ab62341 1:200 12) Rhodaminephalloidin - Invitrogen/ThermoFisher R415 1:200 13) GROα Mouse MAb R&D systems Abingdon, UK MAB275 / clone 20326 1:100 14) IL-8 Goat pAb R&D systems AF-208-NA 1:100 15) Anti-alpha adaptin Mouse mAb BD Transduction labs 610502 1:1000 (WB) 16) GAPDH (control : 36kDa) Mouse mAb Merck Millipore MAB374, clone 6C5 1:5000 (WB) 17) Fluorophore coupled 2 0 various Jackson ImmunoResearch Europe (Newmarket, UK) Alexa 488 AffiniPure Donkey Anti-Mouse IgG (715-545-150) Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Mouse IgG (115-295-003) CyTM5 AffiniPure Donkey Anti- Rabbit IgG (711-175-152) Alexa 488 AffiniPure Donkey Anti-Rabbit IgG (711-545-152) Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (711-295-152) Alexa 488 AffiniPure Donkey Anti-Sheep IgG (713-545-147) 1:1000 Validation key (numbers as in Supplementary Table 1). .. Anti-Human VWF (A0082) has been validated for detection of von Willebrand factor in endothelial cells, megakaryocytes and platelets when tested on formalin-fixed, paraffin-embedded normal human tissues (bone marrow, kidney, liver, lung, lymph node, skin and spleen) as described on the Agilent website https://www.agilent.com/en/product/dako-omnis-solution-for-ihc-ish/primary- antibodies-for-dako-omnis/primary-antibodies-(flex-ready-to-use)/von-willebrand-factor-(dakoomnis)-76216 ).
Immunocytochemistry:Article Title: P-selectin mobility undergoes a sol-gel transition as it diffuses from exocytosis sites into the cell membrane
Article Snippet: .. Antibody reagents and Validation Species Manufacturer Clone / catalogue number Dilutions used for ICC or WB 1) VWF Rabbit pAb DAKO/Agilent Santa Clara, CA USA A0082 1:10000 2) VWF Sheep pAb Serotec Raleigh, NC, USA AHP062 1:10000 3) P-selectin Mouse pAb Bio-Rad (formerly AbD Serotec) AK6/MCA796 1:50 4) Control mouse IgG Mouse pAb Invitrogen/ThermoFisher 16-4714-82 1:50 5) Heparan sulphate Rabbit mAb Sigma-Aldrich MAB1948P/A7L6 1:500 6) Chondroitin-4sulphate Mouse mAb Sigma-Aldrich MAB2030/clone BE-123 1:50 7) FITC-WGA - Vector Labs Inc Burlingame, CA, USA FL-1021 1:400 8) anti-GFP Rabbit mAb Invitrogen/ThermoFisher A-6455 1:300 9) anti-GFP Sheep pAb Bio-Rad Labs Watford, UK 4745-1051 1:250 10) AP2alpha Mouse mAb Invitrogen/ThermoFisher AP6/ MA1-064 1:400 11) anti RFP Rabbit pAb Abcam Cambridge, UK ab62341 1:200 12) Rhodaminephalloidin - Invitrogen/ThermoFisher R415 1:200 13) GROα Mouse MAb R&D systems Abingdon, UK MAB275 / clone 20326 1:100 14) IL-8 Goat pAb R&D systems AF-208-NA 1:100 15) Anti-alpha adaptin Mouse mAb BD Transduction labs 610502 1:1000 (WB) 16) GAPDH (control : 36kDa) Mouse mAb Merck Millipore MAB374, clone 6C5 1:5000 (WB) 17) Fluorophore coupled 2 0 various Jackson ImmunoResearch Europe (Newmarket, UK) Alexa 488 AffiniPure Donkey Anti-Mouse IgG (715-545-150) Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Mouse IgG (115-295-003) CyTM5 AffiniPure Donkey Anti- Rabbit IgG (711-175-152) Alexa 488 AffiniPure Donkey Anti-Rabbit IgG (711-545-152) Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (711-295-152) Alexa 488 AffiniPure Donkey Anti-Sheep IgG (713-545-147) 1:1000 Validation key (numbers as in Supplementary Table 1). .. Anti-Human VWF (A0082) has been validated for detection of von Willebrand factor in endothelial cells, megakaryocytes and platelets when tested on formalin-fixed, paraffin-embedded normal human tissues (bone marrow, kidney, liver, lung, lymph node, skin and spleen) as described on the Agilent website https://www.agilent.com/en/product/dako-omnis-solution-for-ihc-ish/primary- antibodies-for-dako-omnis/primary-antibodies-(flex-ready-to-use)/von-willebrand-factor-(dakoomnis)-76216 ).
Western Blot:Article Title: P-selectin mobility undergoes a sol-gel transition as it diffuses from exocytosis sites into the cell membrane
Article Snippet: .. Antibody reagents and Validation Species Manufacturer Clone / catalogue number Dilutions used for ICC or WB 1) VWF Rabbit pAb DAKO/Agilent Santa Clara, CA USA A0082 1:10000 2) VWF Sheep pAb Serotec Raleigh, NC, USA AHP062 1:10000 3) P-selectin Mouse pAb Bio-Rad (formerly AbD Serotec) AK6/MCA796 1:50 4) Control mouse IgG Mouse pAb Invitrogen/ThermoFisher 16-4714-82 1:50 5) Heparan sulphate Rabbit mAb Sigma-Aldrich MAB1948P/A7L6 1:500 6) Chondroitin-4sulphate Mouse mAb Sigma-Aldrich MAB2030/clone BE-123 1:50 7) FITC-WGA - Vector Labs Inc Burlingame, CA, USA FL-1021 1:400 8) anti-GFP Rabbit mAb Invitrogen/ThermoFisher A-6455 1:300 9) anti-GFP Sheep pAb Bio-Rad Labs Watford, UK 4745-1051 1:250 10) AP2alpha Mouse mAb Invitrogen/ThermoFisher AP6/ MA1-064 1:400 11) anti RFP Rabbit pAb Abcam Cambridge, UK ab62341 1:200 12) Rhodaminephalloidin - Invitrogen/ThermoFisher R415 1:200 13) GROα Mouse MAb R&D systems Abingdon, UK MAB275 / clone 20326 1:100 14) IL-8 Goat pAb R&D systems AF-208-NA 1:100 15) Anti-alpha adaptin Mouse mAb BD Transduction labs 610502 1:1000 (WB) 16) GAPDH (control : 36kDa) Mouse mAb Merck Millipore MAB374, clone 6C5 1:5000 (WB) 17) Fluorophore coupled 2 0 various Jackson ImmunoResearch Europe (Newmarket, UK) Alexa 488 AffiniPure Donkey Anti-Mouse IgG (715-545-150) Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Mouse IgG (115-295-003) CyTM5 AffiniPure Donkey Anti- Rabbit IgG (711-175-152) Alexa 488 AffiniPure Donkey Anti-Rabbit IgG (711-545-152) Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (711-295-152) Alexa 488 AffiniPure Donkey Anti-Sheep IgG (713-545-147) 1:1000 Validation key (numbers as in Supplementary Table 1). .. Anti-Human VWF (A0082) has been validated for detection of von Willebrand factor in endothelial cells, megakaryocytes and platelets when tested on formalin-fixed, paraffin-embedded normal human tissues (bone marrow, kidney, liver, lung, lymph node, skin and spleen) as described on the Agilent website https://www.agilent.com/en/product/dako-omnis-solution-for-ihc-ish/primary- antibodies-for-dako-omnis/primary-antibodies-(flex-ready-to-use)/von-willebrand-factor-(dakoomnis)-76216 ).
Control:Article Title: P-selectin mobility undergoes a sol-gel transition as it diffuses from exocytosis sites into the cell membrane
Article Snippet: .. Antibody reagents and Validation Species Manufacturer Clone / catalogue number Dilutions used for ICC or WB 1) VWF Rabbit pAb DAKO/Agilent Santa Clara, CA USA A0082 1:10000 2) VWF Sheep pAb Serotec Raleigh, NC, USA AHP062 1:10000 3) P-selectin Mouse pAb Bio-Rad (formerly AbD Serotec) AK6/MCA796 1:50 4) Control mouse IgG Mouse pAb Invitrogen/ThermoFisher 16-4714-82 1:50 5) Heparan sulphate Rabbit mAb Sigma-Aldrich MAB1948P/A7L6 1:500 6) Chondroitin-4sulphate Mouse mAb Sigma-Aldrich MAB2030/clone BE-123 1:50 7) FITC-WGA - Vector Labs Inc Burlingame, CA, USA FL-1021 1:400 8) anti-GFP Rabbit mAb Invitrogen/ThermoFisher A-6455 1:300 9) anti-GFP Sheep pAb Bio-Rad Labs Watford, UK 4745-1051 1:250 10) AP2alpha Mouse mAb Invitrogen/ThermoFisher AP6/ MA1-064 1:400 11) anti RFP Rabbit pAb Abcam Cambridge, UK ab62341 1:200 12) Rhodaminephalloidin - Invitrogen/ThermoFisher R415 1:200 13) GROα Mouse MAb R&D systems Abingdon, UK MAB275 / clone 20326 1:100 14) IL-8 Goat pAb R&D systems AF-208-NA 1:100 15) Anti-alpha adaptin Mouse mAb BD Transduction labs 610502 1:1000 (WB) 16) GAPDH (control : 36kDa) Mouse mAb Merck Millipore MAB374, clone 6C5 1:5000 (WB) 17) Fluorophore coupled 2 0 various Jackson ImmunoResearch Europe (Newmarket, UK) Alexa 488 AffiniPure Donkey Anti-Mouse IgG (715-545-150) Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Mouse IgG (115-295-003) CyTM5 AffiniPure Donkey Anti- Rabbit IgG (711-175-152) Alexa 488 AffiniPure Donkey Anti-Rabbit IgG (711-545-152) Rhodamine RedTM-X (RRX) AffiniPure Donkey Anti-Rabbit IgG (711-295-152) Alexa 488 AffiniPure Donkey Anti-Sheep IgG (713-545-147) 1:1000 Validation key (numbers as in Supplementary Table 1). .. Anti-Human VWF (A0082) has been validated for detection of von Willebrand factor in endothelial cells, megakaryocytes and platelets when tested on formalin-fixed, paraffin-embedded normal human tissues (bone marrow, kidney, liver, lung, lymph node, skin and spleen) as described on the Agilent website https://www.agilent.com/en/product/dako-omnis-solution-for-ihc-ish/primary- antibodies-for-dako-omnis/primary-antibodies-(flex-ready-to-use)/von-willebrand-factor-(dakoomnis)-76216 ).
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